Bio-Techne - RnD Systems, CELLXVIVO HUMAN M1 MACROPHAGE DIFFERENTIATION KIT (1 KIT), CDK012

CellXVivo Human M1 Macrophage Differentiation Kit (1 Kit), CDK012 is a manufacturer-supplied cell culture kit for differentiation of human CD14+ monocytes into M1 (pro-inflammatory) macrophages for research use. The kit provides optimized serum-free base media, recombinant human GM‑CSF, and reconstitution buffer to support a 6‑day differentiation protocol and downstream verification by flow cytometry or cytokine ELISA. Primary use cases include generation of defined M1 macrophage populations for functional assays, phenotype analysis, and cytokine secretion studies in immunology and inflammation research.

Key Features:

• Kit format: 1 Kit, catalog CDK012
• Species: Human (for differentiation of CD14+ monocytes into M1 macrophages)
• Contents: Serum‑Free Base Media, Recombinant Human GM‑CSF, Reconstitution Buffer 2
• Yield/Scale: sufficient reagents for differentiation of two 24‑well plates or 5 x 10^7 cells
• Differentiation timeline: 6 days (with downstream stimulation and analysis options)
• Storage: unopened product -20 to -70 °C; opened/reconstituted components 2–8 °C short term

Advantages:

• Optimized serum‑free base media improves efficiency and purity of M1 macrophage differentiation
• Generates M1 macrophages with a CD14+ CD80+ CD163dim CD206+ phenotype suitable for phenotypic and functional assays
• Validated, straightforward procedures that do not require specialized instrumentation
• Includes recombinant human GM‑CSF and necessary buffers to streamline protocol setup