Quantabio QSCR XLT 1STEP RTQPCR TM L-ROX 95134-500

qScript XLT One-Step RT-qPCR ToughMix, Low ROX is a ready-to-use master mix for one-step reverse transcription quantitative PCR (RT-qPCR) that performs first-strand cDNA synthesis and PCR amplification in a single tube. It is formulated for highly sensitive quantification of RNA templates using fluorogenic probe detection chemistries and includes a Low ROX passive reference dye for instruments that require reduced reference signal. Primary use cases include sensitive viral RNA detection, low-abundance transcript quantification, and high-throughput gene expression studies.

Key Features:

• Format: ready-to-use, 2X master mix for one-step RT-qPCR
• Passive reference dye: Low ROX
• Pack size: 500 x 20 µL reactions
• Detection chemistries: compatible with fluorogenic hybridization probe assays (e.g., TaqMan hydrolysis probes)
• Performance: tolerant to common PCR inhibitors and suitable for fast or standard cycling
• Multiplexing: supports detection of up to four targets

Advantages:

• High sensitivity for detection of low-abundance RNA targets and RNA viruses
• Robust performance in the presence of common inhibitors from complex sample matrices
• Flexible cycling: compatible with fast and standard qPCR protocols to support high-throughput workflows
• Low ROX formulation enables use on instruments requiring reduced passive reference signal while maintaining accurate normalization
• Multiplex-capable chemistry allows simultaneous quantification of multiple targets in a single reaction