Quantabio QSCRIPT XLT 1STEP RT-QPCR TOUGHMIX 95132-500

qScript XLT 1-Step RT-qPCR ToughMix is a ready-to-use 2X master mix for one-step reverse transcription quantitative PCR (RT-qPCR) of RNA templates. It combines an engineered XLT reverse transcriptase with a hot-start, highly processive thermostable DNA polymerase in a ToughMix formulation to deliver high sensitivity and reliable amplification in single-plex or multiplex RT-qPCR applications, including assays using fluorogenic hydrolysis probes.

Key Features:

• Format: 2X ready-to-use One-Step RT-qPCR ToughMix
• Enzyme system: qScript XLT reverse transcriptase with hot-start thermostable DNA polymerase
• Inhibitor tolerance: overcomes common inhibitors (polysaccharides, heme, humic acid, melanin)
• Probe compatibility: fluorogenic probe chemistries including TaqMan hydrolysis probes
• Cycling: suitable for fast or standard qPCR cycling conditions
• Multiplexing: supports detection of up to four targets
• Manufacturer part code: 95132-500

Advantages:

• High sensitivity and broad dynamic range for reliable detection from low-abundance RNA
• Robust performance in presence of common PCR inhibitors, enabling use with challenging sample types
• Versatile workflow compatibility—supports fast cycling and multiplex assays with probe-based detection